ACADEMIC AND RESEARCH PEER-REVIEWED MEDICAL JOURNALISSN 1727-2378 (Print)         ISSN 2713-2994 (Online)
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Blood Oxidative-Antioxidant Status of Newborns in Case of Caesarean Section: Accelerated Recovery Program

DOI:10.31550/1727-2378-2021-20-1-45-49
For citation: Medzhidova D.R., Shifman E.M., Cherkesova A.U., Cherkesova D.U. Blood Oxidative-Antioxidant Status of Newborns in Case of Caesarean Section: Accelerated Recovery Program. Doctor.Ru. 2021; 20(1): 45–49. (in Russian). DOI: 10.31550/1727-2378-2021-20-1-45-49
12 March 07:39

Study Objective: To perform a comparative analysis of oxidative-antioxidant status and protein oxidation susceptibility in newborns in case of caesarean section (CS) when the maternity patients are enrolled into an accelerated recovery program (ARP) or with traditional perioperative follow-up.

Materials and Methods. Two groups of newborns were examined: group I (controls, n = 38) — newborns in case of CS and traditional perioperative follow-up, when maternity patients were treated with antibiotics perioperatively, once umbical was crossclamped, with at least 8h preoperative fasting; group II (comparison group, n = 43) — newborns in case of CS and ARP perioperative follow-up, with a carbohydrate drink 2 hours prior to surgery and an antibiotic (cefazolin 2g) administered 30 minutes before surgery. Lipid hydroperoxides (LH), malondialdehyde, vitamin E, SH- and carbonyl group proteins were measured in umbilical blood plasma and red-cell membranes.

Study Results. It was found out that newborns in both groups had basic plasma LH levels higher than in red-cell membranes; and malondialdehyde level in red-cell membranes was significantly higher than in blood plasma (р < 0.05). Accelerated recovery method in CS did not impact LH and malondialdehyde concentration. Unlike maternal patients, newborns in both groups did not demonstrate any signs of oxidative stress. The accelerated recovery method activated thiol antioxidant protection in newborns, resulting in reduction of SH-group level from 66.33 (13.93) to 59.74 (9.44) nmol/mL. Superficial and concealed SH- and carbonyl protein groups were unaffected by the perioperative follow-up method and did not differ between the groups.

Conclusion. Comparison of the oxidative-antioxidant system of the umbical blood of CS newborns with traditional or ARP follow-up allows concluding that newborns in both groups do not demonstrate any signs of oxidative stress. High antioxidant protection in both blood pools is probably a factor limiting oxidative stress.

Contributions: Medzhidova, D.R. — study design, clinical materials collection, patient examination, data processing, analysis and interpretation, statistical data processing; Shifman, E.M. — text of the article, statistical data processing, review of critically important material, approval of the manuscript for publication; Cherkesova, A.U. — thematic publications reviewing, manuscript preparation; Cherkesova, D.U. — thematic publications reviewing, manuscript preparation, data analysis and interpretation.

Conflict of interest: The authors declare that they do not have any conflict of interests.

D.R. Medzhidova — Dagestan State Medical University (a Federal Government-funded Educational Institution of Higher Education), Russian Federation Ministry of Science and Higher Education; 1 Lenin Square, Republic of Dagestan, Makhachkala, Russian Federation 367000. https://orcid.org/0000-0002-6182-9942. E-mail: dzhamilya-med@mail.ru

E.M. Shifman (Corresponding author) — M.F. Vladimirsky Moscow Regional Clinical Research Institute; 61/2 Shchepkin St., Moscow, Russian Federation 129110. https://orcid.org/0000-0002-6113-8498. E-mail: eshifman@mail.ru

A.U. Cherkesova — Dagestan State Medical University (a Federal Government-funded Educational Institution of Higher Education), Russian Federation Ministry of Science and Higher Education; 1 Lenin Square, Republic of Dagestan, Makhachkala, Russian Federation 367000. https://orcid.org/0000-0002-3710-655X. E-mail: asniyatcher@gmail.com

D.U. Cherkesova — Dagestan State Medical University (a Federal Government-funded Educational Institution of Higher Education), Russian Federation Ministry of Science and Higher Education; 1 Lenin Square, Republic of 

Доктор.ру

Table 1

Lipid hydroperoxides and malondialdehyde levels in newborn blood plasma and red-cell membranes, M (SD)

t8_1.jpg

Notes.

  1. Differences in lipid hydroperoxides and malondialdehyde levels in newborn blood plasma and red-cell membranes in controls and comparison group is statistically significant (р < 0.05).
  2. Units of measurement for lipid hydroperoxides: in plasma — nmol/L; in red-cell membranes — nmol/mg of protein; units of measurement for malondialdehyde — µmol/L.

Table 2
SH groups in blood plasma proteins and red-cell membranes of newborn umbical blood, M (SD)

t8_2.jpg

* Statistically significant difference from control group (р < 0.05). 

Table 3
Vitamin E levels in blood plasma and red-cell membranes of newborns, µmol/L, M (SD)

t8_3.jpg

* Differences in blood plasma and red-cell membrane are statistically significant (р < 0.05).

Table 4
Carbonyl group levels in blood plasma and red-cell membranes of newborns, nmol/mg of protein, M(SD)

t8_4.jpg

Received: 02.12.2020
Accepted: 18.01.2021

12 March 07:39
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