A PEER-REVIEWED JOURNAL OF RESEARCH AND CLINICAL MEDICINEISSN 1727-2378 (Print)         ISSN 2713-2994 (Online)
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Microbiota and Local Immunity of Vaginal Mucosa in Very Young Girls: Normal and Abnormal Parameters

For citation: Uvarova Ye. V., Batyrova Z. K., Kumykova Z. Kh., Donnikov A. Ye., Burmenskaya O. V., Namazova-Baranova L. S. Microbiota and Local Immunity of Vaginal Mucosa in Very Young Girls: Normal and Abnormal Parameters. Doctor.Ru. 2017; 3(132): 59–65.
14 april 2017

Study Objective: To describe the microbiota and local immunity of the vaginal mucosa in very young girls with recurrent labial adhesions, acute bacterial vulvovaginitis, or atopic dermatitis, compared to age-matched healthy girls.

Materials and Methods: The study included 217 girls, aged one to 36 months: 68 with acute bacterial vulvovaginitis, 60 with recurrent labial adhesions, 27 with atopic dermatitis, and 62 healthy girls with no abnormalities in physical or sexual development (control group). Quantitative molecular genetic analysis was performed on epithelial scrapes of the vaginal vestibule and vaginal wall obtained from all participants, to assess microbial composition (using real-time polymerase chain reaction) and local immunity.

Study Results: The following groups of anaerobic microorganisms were the most commonly observed components of the bacterial microbiota in very young girls: Eubacterium spp., Prevotella bivia/Porphyromоnas spp., Megasphaera spp./Velionella spp./Dialister, and Peptostreptococcus spp. Girls with labial adhesions frequently (about 1.2 times more so than healthy girls) had Mobiluncus spp./Corynebacterium spp (100% vs. 83%; р = 0.018). In girls with acute vulvovaginitis, the Enterococcus and Gardnerella vaginalis genomes were found less frequently by factors of 2.6 (27.3% vs. 72.3%; р = 0.014) and 3.2 (18.2% vs. 59.6%; р = 0.033), respectively, than in the control group. The Lactobacillus spp./G. vaginalis ratio was elevated because of a greater percentage of Lactobacillus spp. genomes. In the group of girls with atopic dermatitis, the G. vaginalis and Lactobacillus spp. genomes were found less frequently by factors of 3.2 (р = 0.001) and 2.4 (р = 0.002), respectively, than in the control group. There were no significant differences, however, in the quantity of any the groups of microorganisms studied, between the groups of girls with different health problems.

In participants with recurrent labial adhesions the following changes were observed in local immunity of the vaginal mucosa: lower levels of both pro-inflammatory (IL-1β, IL-10, and IL-12α) and anti-inflammatory (IL-18) cytokines and lower expression of messenger ribonucleic acid (mRNA), transforming growth factor β, and tumor necrosis factor α. Changes observed in samples taken from girls with vulvovaginitis included a 1.7 times higher expression of CD45 mRNA and an expression of IL-18 that was lower by a factor of 1.9 than in the control group. Girls with atopic dermatitis had lower expression of IL-8, IL-10, and CD45 mRNA than the age-matched healthy controls.

Conclusion: Recurrent labial adhesions in very young girls are not associated with acute inflammation of infectious origin. The qualitative composition of the microbiota of the vaginal mucosa in this age population changes over time, with shifts being observed in different groups of organisms. It is possible that the balance of vaginal microbiota in these girls is maintained by the presence of certain groups of microorganisms in very low quantities. Low quantities of G. vaginalis also play an important regulatory role.

Ye. V. Uvarova — V. I. Kulakov Research Center for Obstetrics, Gynecology and Perinatology, Moscow. E-mail: elena-uvarova@yandex.ru

Z. K. Batyrova — V. I. Kulakov Research Center for Obstetrics, Gynecology and Perinatology, Moscow. E-mail: linadoctor@mail.ru

Z. Kh. Kumykova — V. I. Kulakov Research Center for Obstetrics, Gynecology and Perinatology, Moscow. E-mail: zai-kumykova@yandex.ru

A. Ye. Donnikov — V. I. Kulakov Research Center for Obstetrics, Gynecology and Perinatology, Moscow. E-mail: a_donnikov@oparina4.ru

O. V. Burmenskaya — V. I. Kulakov Research Center for Obstetrics, Gynecology and Perinatology, Moscow. E-mail: o_bourmenskaya@oparina4.ru

L. S. Namazova-Baranova — Scientific Centre for Children's Health, Moscow. I. M. Sechenov First Moscow State Medical University. N. I. Pirogov Russian National Research Medical University, Moscow. E-mail: namazova@nczd.ru


Доктор.ру
14 April 00:00
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