ACADEMIC AND RESEARCH PEER-REVIEWED MEDICAL JOURNALISSN 1727-2378 (Print)         ISSN 2713-2994 (Online)
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In vitro Sensitivity of Vaginal Microorganisms to a Combination Intravaginal Antimicrobial Preparation Based on Metronidazole and Clotrimazole

DOI:10.31550/1727-2378-2019-162-7-65-68
Bibliography link: Savicheva A.M., Shipitsyna E. V., Spasibova E.V. In vitro Sensitivity of Vaginal Microorganisms to a Combination Intravaginal Antimicrobial Preparation Based on Metronidazole and Clotrimazole. Doctor.Ru. 2019; 7(162): 65–68. DOI: 10.31550/1727-2378-2019-162-7-65-68

Study Objective: To determine the in vitro sensitivity of microorganisms isolated from the vaginas of women of reproductive age to a combination topical preparation based on metronidazole and clotrimazole.

Study Design: This was an experimental study.

Materials and Methods: A total of 421 isolates of microorganisms were evaluated in the study, including Candida (Candida albicans, C. glabrata); bacteria associated with bacterial vaginosis (BV) (Gardnerella vaginalis, Atopobium vaginae); Gram-negative bacteria (Escherichia coli, Klebsiella pneumoniae, Enterobacter spp.); Gram-positive bacteria (Streptococcus agalactiae, Enterococcus spp.); and lactobacilli (Lactobacillus iners, L. jensenii, L. gasseri, L. crispatus). Microbial sensitivity to the study preparation was assessed using Mueller-Hinton agar. The study drug was applied with a 10 μL calibrated loop. Plates were read visually by measuring the diameters of zones of inhibition after incubation for 24 hours at 37°С.

Study Results: All of the isolates tested from the two species of the genus Candida, G. vaginalis, and A. vaginae were sensitive to the study drug. The sensitivity of the various Lactobacillus species differed considerably. All of the isolates from the Gram-negative and Gram-positive bacteria were resistant to the study drug.

Conclusion: The intravaginal antimicrobial preparation based on metronidazole and clotrimazole examined in this study is highly active in vitro against the key microorganisms responsible for BV (G. vaginalis and A. vaginae) and Candida vulvovaginitis (C. albicans and C. glabrata), and has no effect on the main Lactobacillus species (L. crispatus and L. iners).

A.M. Savicheva — D.O. Ott Research Institute of Obstetrics, Gynecology, and Reproductive Medicine; 3 Mendeleevskaya Liniya, St. Petersburg, Russian Federation 199034. St. Petersburg State Pediatric Medical University, Russian Ministry of Health; 2 Litovskaya St., St. Petersburg, Russian Federation 194100. eLIBRARY.RU SPIN: 8007-2630. E-mail: savitcheva@mail.ru

E.V. Shipitsyna — D.O. Ott Research Institute of Obstetrics, Gynecology, and Reproductive Medicine; 3 Mendeleevskaya Liniya, St. Petersburg, Russian Federation 199034. St. Petersburg State Pediatric Medical University, Russian Ministry of Health; 2 Litovskaya St., St. Petersburg, Russian Federation 194100. eLIBRARY.RU SPIN: 7660-7068. E-mail: shipitsyna@inbox.ru

E.V. Spasibova — D.O. Ott Research Institute of Obstetrics, Gynecology, and Reproductive Medicine; 3 Mendeleevskaya Liniya, St. Petersburg, Russian Federation 199034. St. Petersburg State Pediatric Medical University, Russian Ministry of Health; 2 Litovskaya St., St. Petersburg, Russian Federation 194100. eLIBRARY.RU SPIN: 3585-8770. E-mail: elena.rybina@gmail.ru

Доктор.ру
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